This is the second article in this series discussing HPLC oligonucleotide separations. This article will discuss the separation of oligonucleotides and dsDNAs using a wide-pore, polymer based reversed ...

The use of acidic MPAs (pH 2–4) is the default in pharmaceutical testing because they suppress silanol ionization (from Si-OH to Si-O -) and improve peak shape for basic drugs. However, highly ...

The gold standard of quality assessment of drugs is nowadays an HPLC using a reversed-phase (RP) column chromatography and aqueous buffers, acetonitrile, and/or methanol as mobile phase, com ...

High performance liquid chromatography, more commonly known by the acronym HPLC, is a method used to identify substances in a mixture. There are several types of HPLC, such as reversed-phase, ...

Any analytical method must strike a balance between its intrinsic scientific potential its capacity for robustness and reliability, particularly in regulated industries. In the case of HPLC, this ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

As you reach the end of the run and possibly the conclusion of the workday, you may wonder what to do with the column you have recently used. You might consider whether it should remain attached to ...