The following picture shows the ethidium bromidestained bands revealed by gel electrophoresis of two different DNA samples digested with two different restriction enzymes. One of the DNAs is human genomic DNA, the other is the small genome of a bacteriophage (bacterial virus) that infects E. coli cells. One of the restriction enzymes is E c o RI(5^' G^∧ A ATTC 3^') ; the other is HpaII(5^' C^∧ CGG 3^') . For each of the four lanes on the gel (A-D), identify which of the two DNA samples was analy
The following picture shows the ethidium bromidestained bands re…
Jun 7, 2020
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DNA strands on a gel stained with ethidium bromide when viewed ... | Filo
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🌾🌾Dr.Agrarian🌾🌾 on Instagram: "Ethidium bromide (EtBr) is an intercalating fluorescent dye used in DNA analysis, especially in agarose gel electrophoresis. Main function EtBr inserts between base pairs of double-stranded DNA. When exposed to UV light, the EtBr–DNA complex fluoresces orange, allowing visualization of DNA bands in the gel. Purpose in DNA analysis Detect and locate DNA fragments after electrophoresis. Estimate DNA size and quantity by comparing band intensity with a DNA ladder.
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