PCR: Steps Involved in Polymerase Chain Reaction
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◾ PCR 🔹Denaturation In the first stage, the denaturation stage, double stranded DNA is separated into two single strands by breaking the hydrogen bonds between the nucleotide base pairs (bp). In a PCR reaction, an initial denaturation step is needed at the beginning of PCR, before the cycling of the three stages begins. This initial denaturation step usually occurs at around 94°C (or in the range of 94-98 C), depending on the specific polymerase involved. To fully denature the DNA, initial dena
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