Using FRAP for Risk Assessment
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WF FRAP Fluorescence Recovery After Photobleaching
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leica-microsystems.com
Diffusion rates of various biomolecules can be measured inside the cell using GFPs in a technique called fluorescence recovery following photobleaching (FRAP). In this approach, a region inside the cell is subjected to intense laser light, which photobleaches any fluorescence in that region. GFPs located outside the exposed region then diffuse back into the bleached region, and this movement is studied using low intensity laser light. What is the hydration radius of a GFP molecule in water at a
Diffusion rates of various biomolecules can be measured in…
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